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rabbit polyclonal anti total aurkb (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc rabbit polyclonal anti total aurkb
$( A ) Phosphorylation of aurora kinases in the presence of selected Vif point mutants. CEM-T4s were transduced with lentiviruses encoding EGFP-P2A-Vif at an MOI of 3, then lysed in 2% SDS and analysed by immunoblot with anti-Vif, anti-phospho-AURK, anti-total <t>AURKB</t> and anti-p97 (loading control) antibodies after 48 hr. Ctrl, control construct encoding EGFP-SBP-ΔLNGFR. ( B ) Regulation of cyclin B1 by selected Vif point mutants in cells from proteomic experiment 1 . For each Vif point mutant, abundance of cyclin B1 is shown as a ratio to the mean abundance in the three mock-transduced samples. Significant outliers from the distribution of abundances in mock-transduced samples are highlighted (see Materials and methods for details). *p<0.05; **p<0.05. ( C–D ) Regulation of cell cycle by selected Vif point mutants. CEM-T4s were transduced with lentiviruses encoding EGFP-P2A-Vif at an MOI of 3, then fixed in 90% methanol, stained with 7-AAD and analysed by flow cytometry after 48 hr. Representative data ( C ) from three biological replicates ( D ) are shown. For each Vif point mutant, the fraction of cells in G2/M is shown as a ratio to the fraction of cells in G2/M in mock-transduced cells. Individual data points reflect biological replicates. Mean values with SEM are indicated. Significant differences compared with mock-transduced cells are highlighted ( t -tests). *p<0.05. Ctrl, control construct encoding EGFP-SBP-ΔLNGFR.$
Rabbit Polyclonal Anti Total Aurkb, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+antibody+to+aurb/Aurora+B%2FAIM1+Antibody/pmc07920553-16-2-7
Average 94 stars, based on 143 article reviews

rabbit polyclonal anti total aurkb - by Bioz Stars, 2026-09

94/100 stars

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1) Product Images from "Antagonism of PP2A is an independent and conserved function of HIV-1 Vif and causes cell cycle arrest"

Article Title: Antagonism of PP2A is an independent and conserved function of HIV-1 Vif and causes cell cycle arrest

Journal: eLife

doi: 10.7554/eLife.53036

$( A ) Phosphorylation of aurora kinases in the presence of selected Vif point mutants. CEM-T4s were transduced with lentiviruses encoding EGFP-P2A-Vif at an MOI of 3, then lysed in 2% SDS and analysed by immunoblot with anti-Vif, anti-phospho-AURK, anti-total AURKB and anti-p97 (loading control) antibodies after 48 hr. Ctrl, control construct encoding EGFP-SBP-ΔLNGFR. ( B ) Regulation of cyclin B1 by selected Vif point mutants in cells from proteomic experiment 1 . For each Vif point mutant, abundance of cyclin B1 is shown as a ratio to the mean abundance in the three mock-transduced samples. Significant outliers from the distribution of abundances in mock-transduced samples are highlighted (see Materials and methods for details). *p<0.05; **p<0.05. ( C–D ) Regulation of cell cycle by selected Vif point mutants. CEM-T4s were transduced with lentiviruses encoding EGFP-P2A-Vif at an MOI of 3, then fixed in 90% methanol, stained with 7-AAD and analysed by flow cytometry after 48 hr. Representative data ( C ) from three biological replicates ( D ) are shown. For each Vif point mutant, the fraction of cells in G2/M is shown as a ratio to the fraction of cells in G2/M in mock-transduced cells. Individual data points reflect biological replicates. Mean values with SEM are indicated. Significant differences compared with mock-transduced cells are highlighted ( t -tests). *p<0.05. Ctrl, control construct encoding EGFP-SBP-ΔLNGFR.$
Figure Legend Snippet: ( A ) Phosphorylation of aurora kinases in the presence of selected Vif point mutants. CEM-T4s were transduced with lentiviruses encoding EGFP-P2A-Vif at an MOI of 3, then lysed in 2% SDS and analysed by immunoblot with anti-Vif, anti-phospho-AURK, anti-total AURKB and anti-p97 (loading control) antibodies after 48 hr. Ctrl, control construct encoding EGFP-SBP-ΔLNGFR. ( B ) Regulation of cyclin B1 by selected Vif point mutants in cells from proteomic experiment 1 . For each Vif point mutant, abundance of cyclin B1 is shown as a ratio to the mean abundance in the three mock-transduced samples. Significant outliers from the distribution of abundances in mock-transduced samples are highlighted (see Materials and methods for details). *p<0.05; **p<0.05. ( C–D ) Regulation of cell cycle by selected Vif point mutants. CEM-T4s were transduced with lentiviruses encoding EGFP-P2A-Vif at an MOI of 3, then fixed in 90% methanol, stained with 7-AAD and analysed by flow cytometry after 48 hr. Representative data ( C ) from three biological replicates ( D ) are shown. For each Vif point mutant, the fraction of cells in G2/M is shown as a ratio to the fraction of cells in G2/M in mock-transduced cells. Individual data points reflect biological replicates. Mean values with SEM are indicated. Significant differences compared with mock-transduced cells are highlighted ( t -tests). *p<0.05. Ctrl, control construct encoding EGFP-SBP-ΔLNGFR.

Techniques Used: Phospho-proteomics, Transduction, Western Blot, Control, Construct, Mutagenesis, Staining, Flow Cytometry

Figure Legend Snippet:

Techniques Used: cDNA Library Assay, Flow Cytometry, Western Blot, Recombinant, Control, Construct, Cloning, Transfection, Sample Prep, Software

Purification:

Article Title: Inhibition of Aurora A in response to DNA damage.
Article Snippet: IgGs were purified by FPLC on a Protein A-Sepharose column (Amersham-Pharmacia). .. The purified polyclonal antibody to AurB (#3094) was from Cell Signaling Technology. ..

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Journal: eLife

Article Title: Antagonism of PP2A is an independent and conserved function of HIV-1 Vif and causes cell cycle arrest

doi: 10.7554/eLife.53036

Figure Lengend Snippet: ( A ) Phosphorylation of aurora kinases in the presence of selected Vif point mutants. CEM-T4s were transduced with lentiviruses encoding EGFP-P2A-Vif at an MOI of 3, then lysed in 2% SDS and analysed by immunoblot with anti-Vif, anti-phospho-AURK, anti-total AURKB and anti-p97 (loading control) antibodies after 48 hr. Ctrl, control construct encoding EGFP-SBP-ΔLNGFR. ( B ) Regulation of cyclin B1 by selected Vif point mutants in cells from proteomic experiment 1 . For each Vif point mutant, abundance of cyclin B1 is shown as a ratio to the mean abundance in the three mock-transduced samples. Significant outliers from the distribution of abundances in mock-transduced samples are highlighted (see Materials and methods for details). *p<0.05; **p<0.05. ( C–D ) Regulation of cell cycle by selected Vif point mutants. CEM-T4s were transduced with lentiviruses encoding EGFP-P2A-Vif at an MOI of 3, then fixed in 90% methanol, stained with 7-AAD and analysed by flow cytometry after 48 hr. Representative data ( C ) from three biological replicates ( D ) are shown. For each Vif point mutant, the fraction of cells in G2/M is shown as a ratio to the fraction of cells in G2/M in mock-transduced cells. Individual data points reflect biological replicates. Mean values with SEM are indicated. Significant differences compared with mock-transduced cells are highlighted ( t -tests). *p<0.05. Ctrl, control construct encoding EGFP-SBP-ΔLNGFR.

Article Snippet: Antibody , Rabbit polyclonal anti-total AURKB , Cell Signalling Technology , Cat. #: 3094 , Immunoblot (1:500).

Techniques: Phospho-proteomics, Transduction, Western Blot, Control, Construct, Mutagenesis, Staining, Flow Cytometry

Journal: eLife

Article Title: Antagonism of PP2A is an independent and conserved function of HIV-1 Vif and causes cell cycle arrest

doi: 10.7554/eLife.53036

Figure Lengend Snippet:

Article Snippet: Antibody , Rabbit polyclonal anti-total AURKB , Cell Signalling Technology , Cat. #: 3094 , Immunoblot (1:500).

Techniques: cDNA Library Assay, Flow Cytometry, Western Blot, Recombinant, Control, Construct, Cloning, Transfection, Sample Prep, Software

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